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Kirby Bauer examination (Antimicrobial sensitivity test)
The Kirby Bauer examination can also be referred to as disk diffusion technique, it is mostly used antibiotic test in the determination of the choice of antibiotics to be employed when treating an infection. The technique depends on the reserve of microbial development tested under normal circumstances.
In the test, a culture medium, precisely Mueller Hinton agar, is consistently and aseptically cultured with the organism to be tested, and then sieve paper floppies, that is soaked with a particular saturation of a certain antibiotic, are positioned on the medium. The microorganism will nurture on the agar plate whereas the antibiotic functions to prevent the development. If the microorganism is vulnerable to a particular antibiotic, no growth will be observed around the disc comprising the antibiotic. Therefore, a region of prevention may be detected and tested to decide the vulnerability to an antibiotic for the specific microorganism. The results are matched to the standards put in place by the Clinical and Laboratory Standards Institute (CLSI). The organism can later be grouped as either Resistant (R), Intermediate (I), or Susceptible (S) Kirby Bauer examination (Antimicrobial sensitivity test).
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Kirby Bauer Test for Checking Antibiotic Sensitivity
Testing Sensitivity to Antibiotics
The Kirby Bauer test, also known as the disk diffusion technique, is a common way to figure out which antibiotics work best for treating infections. This method relies on studying how bacteria grow under normal conditions.
In this test, a special substance called Mueller Hinton agar is spread on a plate. Then, the bacteria to be tested are carefully grown on this plate. Small pieces of paper soaked in a specific antibiotic are placed on the plate. While the bacteria grow on the plate, the antibiotic stops their growth. If the bacteria are affected by the antibiotic, there won’t be any growth around the paper soaked in that antibiotic. This helps figure out if the bacteria are sensitive to that antibiotic. The results are compared to the standards set by the Clinical and Laboratory Standards Institute (CLSI). Based on these results, the bacteria can be categorized as Resistant (R), Intermediate (I), or Susceptible (S) in terms of their sensitivity to antibiotics.
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